Effect of glucose availability on glucose transport in bovine mammary epithelial cells

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Effect of glucose availability on glucose transport in bovine mammary epithelial cells.

Primary bovine mammary epithelial cells (BMEC) were cultured in media containing varying concentrations of glucose, to determine the effects of glucose availability on glucose transport and its mechanism in bovine mammary gland. The BMEC incubated with 10 and 20 mM glucose had twofold greater glucose uptake than that with 2.5 mM glucose (P < 0.05). Increased glucose availability enhanced the ce...

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Effects of Glucose Availability on Expression of the Key Genes Involved in Synthesis of Milk Fat, Lactose and Glucose Metabolism in Bovine Mammary Epithelial Cells

As the main precursor for lactose synthesis, large amounts of glucose are required by lactating dairy cows. Milk yield greatly depends on mammary lactose synthesis due to its osmoregulatory property for mammary uptake of water. Thus, glucose availability to the mammary gland could be a potential regulator of milk production. In the present study, the effect of glucose availability on expression...

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Interaction of primary mammary bovine epithelial cells with biofilm-forming staphylococci associated with subclinical bovine mastitis

Background: Staphylococci are recognized worldwide as one of the most important etiological agents of bovine mastitis due to their virulence factors such as their ability to penetrate inside mammary epithelial cells and their ability to form biofilm. Aims: The objectives of this study were to establish a model of primary mammary epithelial cells origin...

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Low glucose enhances Na+/glucose transport in bovine brain artery endothelial cells.

BACKGROUND AND PURPOSE Brain arteries are structurally characterized by the tight junctions of the endothelium and by no vasa vasorum that feed arteries themselves. This raises the question of how brain arteries are provided with glucose. A possible explanation is that glucose uptake into arteries may be mediated by both GLUT1, a facilitative glucose transporter, and a Na+/glucose cotransporter...

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Effects of glucose on lactose synthesis in mammary epithelial cells from dairy cow.

BACKGROUND Lactose, as the primary osmotic component in milk, is the major determinant of milk volume. Glucose is the primary precursor of lactose. However, the effect of glucose on lactose synthesis in dairy cow mammary glands and the mechanism governing this process are poorly understood. RESULTS Here we showed that glucose has the ability to induce lactose synthesis in dairy cow mammary ep...

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ژورنال

عنوان ژورنال: Animal

سال: 2012

ISSN: 1751-7311

DOI: 10.1017/s1751731111001893